例句與造句

1. Material and methods normal rats of male sd were divided into young , adult , and aging groups . preparation of samples for light microscopy : animals were anesthetized by peritoneal injection of 6 % chloral hydrate ( 0 . 5ml / 100g body weight ) . perfusion and fixation of animals were carried out by a common procedure : 37 normal saline 50 - 100 ml and then 4 % paraformaldehyde pbs 100 - 400ml were perfused through the left ventricle of the heart , the whole procedure was lasted for about somin . the entire brain was dissected out and dipped in the fixative solution for 12h at 4 . brain pieces targeted were choosen and then passed the graded alcohols for dehydration , dipping into paraffin for embeding , and reshaping the pieces
   2 ）磷酸緩沖液100400m ， 30分鐘灌注完畢，取出整腦，在上述固定劑oc ）內(nèi)后固定12小時。切取觀察部位腦塊，然后，進行梯度酒精脫水，浸蠟，包埋，修塊，石蠟連續(xù)切片（德國leica石蠟切片機人切片厚度still ， zlllll ，蛋自甘油載片撈片， 60c烤箱過夜，二甲苯脫蠟，梯度酒精置換，浸水， h六染色，梯度酒精脫水，二甲苯透明，中性樹脂封片。室溫風干后，顯微鏡觀片， olympus萬能顯微鏡照相。
2. It's difficult to find fixative solution in a sentence. 用fixative solution造句挺難的